Protein Purification Systems

PureTec® Tangential Flow Filtration (TFF)

Cross-Flow Filtration

Tangential Flow Filtration Protein Separations Purification Vessels Crossflow Filtration System Data Collection TFF System

PureTec Advantage:

Walk-away TFF automation includes operational safety alarms and automated shutdown when filtration benchmarks have been achieved. PureTec maintains user-selected trans-membrane pressure during TFF / Cross-Flow Filtration / Protein Purification operations.

  • PureTec Tangential Flow Filtration System automates concentration & diafiltration of biopharmceutical solutions. A 500 ml retentate vessel (polysulfone) with magnetic stirrer is included; 50 ml and 1000 ml retentate vessels are available options. Programmable peristaltic system generates a flow rate up 4.0 liters/minute with high precision.
  • PureTec TFF System includes three (3), disposable pressure sensors for monitoring feed, retentate and permeate line pressure. PureTec automatically maintains selected trans-membrane pressure.
  • PureTec TFF System includes an electronic scale for monitoring permeate collection rate. Optional printer documents up to 11 filtration parameter including date/time stamp.

PureTec® + SciDoc = Automated Documentation

The SciDoc Documentation Package, used in conjunction with the PureTec, displays, calculates compiles, and documents 15 filtration parameters; providing you with an instantaneous overview of your filtration progress and status. This Excel based application also automatically populates graphs in real time. The PureTec gives you the information & control you need to optimize your filtration for maximized yield.

Crossflow Filtration System Data Collection
Example Data Collection Screen Shot
Documented Filtration Parameters:
  • Time: HH:MM:SS
  • Permeate Weight
  • Feed Line Pressure
  • Retentate Line Pressure
  • Permeate Line Pressure
  • Trans-Membrane Pressure
  • Feed Flow Rate
  • Permeate Flow Rate
  • Retentate Flow Rate
  • Permeate Flux
  • Concentration Factor
  • Operation Status: RUN, STOP
  • Operational Alarms
  • Monitor 1 Output
  • Monitor 2 Output

SciLog PureTec Compatible Filter Cartridges

The PureTec can be used with a variety of TFF Membrane and Hollow Fiber filter sytems, from several manufactures, i.e. Pall, Sartorius, Millipore, etc. If your flow rates will exceed 2000 ml/min, the SciPure is capable of these higher flow rates.

 

 

Product Literature

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AccuTec™ Manually Controlled Tangential Flow Filtration (TFF) Lab Protein Purification System Tangential Flow Filtration

AccuTec Manual Tangential Flow Filtration System

Manual Crossflow Filtration System Manual Protein Purification System Cross-Flow Filtration System Tangential Flow Filtration System TFF System

The ACCUTec TFF System is perfect for starting volumes of 50 ml to 5000 ml and has very low hold-up volumes. All wetted surfaces are low protein binding, chemically resistant materials.

AccuTec TFF System Includes:

  • Compact, High-Precision ACCU peristaltic pump: Choice of three different motor sizes and multiple different tubing sizes can be used. Can add a second Tandem peristaltic head (piggy-back) to increase flow or eliminate pulsation.
  • Gentle Sample Processing: low-shear pumping action with Tandem™ peristaltic 4-roller pump head.
  • Aseptic Reservoir Vessel: Closed system creates vacuum in reservoir to draw process solution in as solution is being drawn out of the reservoir by the pump. Graduated 500 ml vessel made of FDA approved polysulfone is compatible with disinfectant, e.g.: isopropyl alcohol or sodium hydroxide. Seals do not make contact with your process solution. All fluid connections are 316 SS Female Luer with SS tubing lining all fluid channels; no exposed connector threads to contaminate. Optional 50 ml vessel is also available (#090-012).
  • Magnetic Stirrer: powerful, variable speed stirrer with LED in stainless steel enclosure. Use with either 50 ml or 500 ml graduated vessel.
    Filter Holder: to hold/organize your Minimate or Pellicon filter cartridge and gauges.
  • Two Pressure Gauges: to monitor your feed and retentate pressure. If you require monitoring a third pressure, this can also be added. Stainless steel with Luer connectors.
  • Fittings/Tubing Start-Up Kit: includes Male and Female Luer fittings, adapters, barbs, multiple plastic valves, stirrer magnet, tees, clamps reservoir air filters, tubing lengths.
  • Easy Clean-up and Maintenance: sent to you ready to use. Eliminate cleaning/ cross-contamination issues by using low-cost replacement components/tubing.

Product Literature

Manuals

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membrane separations Crossflow Filtration System cross-flow filtration filtration